Journal: Oncology Reports

Article Title: Tumor associated macrophages induce epithelial to mesenchymal transition via the EGFR/ERK1/2 pathway in head and neck squamous cell carcinoma

doi: 10.3892/or.2018.6657

Figure Lengend Snippet: Induction and acquirement of macrophage polarization. Macrophages with M0, M1 and M2 functional profiles were acquired from THP1 cells following treatment with PMA, PMA/LPS/IFN-γ and PMA/IL-4/IL-13, respectively. (A) Expression of classical M0, M1 and M2 polarized macrophage surface markers (CD68 and CD163) was evaluated by flow cytometry. (B) mRNA expression levels of TNF-α, IL-1β, TGF-β and IL-10 in differentiated cells following induction were detected by reverse transcription-quantitative polymerase chain reaction. Data are expressed as the mean ± standard error of the mean. *P<0.05 and ***P<0.001 vs. THP1. PMA, phorbol-12-myristate-13-acetate; LPS, lipopolysaccharide; CD, cluster of differentiation; IFN-γ, interferon-γ; IL, interleukin; TNF-α, tumor necrosis factor-α; TGF-β, transforming growth factor-β.

Article Snippet: THP1 [human acute monocytic leukemia cell line; China Center for Type Culture Collection (CCTCC), Wuhan, China] cells were maintained in RPMI-1640 (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Cal27 (oral tongue squamous carcinoma cell line; CCTCC) cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc.).

Techniques: Functional Assay, Expressing, Flow Cytometry, Reverse Transcription, Real-time Polymerase Chain Reaction